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Imaging Flow Cytometry Core Facility

 

 

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Amnis Image Stream - Multispectral Imaging Flow Cytometer

Imaging Flow Cytometry combines speed, fluorescence sensitivity and phenotyping abilities of traditional flow cytometry with detailed imagery and functional insight of fluorescence microscopy in one machine. This unique technique allows a discrimination of cells objectively and statistically based on their appearance such as shape, size, texture, signal strength, location.

Overcoming the limitations of both techniques, the Imaging Flow Cytometer opens the door to a range of novel applications such as Translocation, Co-localization, Immune synapse studies, Shape change and Chemotaxis, Internalization, Stem cell differentiation and many more.

The Image Stream Core Facility is equipped with the most advanced Imaging Flow cytometer on the market. It is augmented with five lasers: 488 nm, 405 nm, 561 nm, 642 nm and SSC (785 nm). Two CCD cameras allow detection in 12 image channels with 3 different magnifications (20x, 40x, 60x). The EDF function extends the depth of field which can improve spot counting applications such as FISH, autophagy and nuclear translocation.

 

 

 

 

 

Data Analysis with IDEAS®

IDEAS® Image analysis software allows post-acquisition compensation and provides a large selection of features and masks to describe and analyse images. Circularity, Perimeter, Bright Detail Similarity, Max/Mean/Min Pixel, Spot area, Thickness are but a few examples.

Quantify the morphological changes of cells following activation or count the number of parasites in cells and distinguish between adherence and invasion of parasites. The IDEAS Application performs statistical analyses on thousands of events and offers visual confirmation of any individual cell or event.

We can guide you through the life-cycle of your project, from experimental setup and result analysis to composing graphics and figures for publication.

Our experienced professional staff offers training and assistance for both imaging and analysis.

 

**Free “Proof-of-concept” run is available upon request. Please contact the Core Facility Operator to schedule.

For more detailed information visit:

Amnis® Imaging Flow Cytometers

 

 Last update: 18 May 2017






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Image Stream Core Facility, Departm. of Women´s Health, Schenke-Layland Group
Silcherstraße 7/1
72076 Tübingen
Operator: Simone Poeschel
Tel. 07071 29-85206 ; 83068
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