M3 Lecture Series
HuLISST – a Human Liver Cell Atlas Integrating Single Cell and High-Resolution Spatial Transcriptomics
We are happy to announce that Prof. Jan Tchorz will give a talk at he M3 Research Center.
On Monday 5.00 pm he will talk about:
HuLISST
Complex organ function relies on the spatial coordination of cellular identities and metabolic programs. In the liver, metabolic programs are segregated along the porto-central axis to maintain systemic homeostasis, yet the status of this spatial logic in human chronic liver disease (CLD) remains unknown. Here, we present the Human Liver Integrated Single-cell and Spatial Transcriptomics (HuLISST) atlas, a high-resolution multimodal reference of the healthy and diseased liver. By developing an entropy-based framework to quantify spatial order, we identify a profound loss of zone-specific metabolic programs and collapse of metabolic zonation across CLD etiologies. Spatial analyses and lineage tracing reveal a metabolic identity switch in peri-injury hepatocytes and transient metabolic reprogramming during biliary-to-hepatocyte transdifferentiation. We find that CLD is characterized by a spatial redistribution of WNT and RSPO ligands, marked by their depletion from the healthy parenchymal gradient and ectopic enrichment within injury niches. This signaling translocation is associated with the loss of pericentral metabolism and the emergence of aberrant hepatocyte identities. Together, our study establishes a high-resolution spatial reference of the diseased human liver and reveals fundamental principles by which spatial signaling and cell plasticity reshape metabolic organization in CLD.
At the M3 Research Center, Otfried-Müller-Str. 37, Level 2, Room 2.208
And as usual you can also take part via Zoom
https://med-uni-tuebingen-de.zoom-x.de/j/69823012125?pwd=U2bdgol1wlH6VXnY0VKpaqJuZBpv02.1
Meeting-ID: 698 2301 2125
Kenncode: 780966
