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Advanced Metabolic Profiling for Biomedical Research
Our Core Facility Metabolomics operates across three laboratories equipped with state-of-the-art instrumentation for targeted, untargeted, quantitative, as well as spatial metabolomics and lipidomics. We offer comprehensive services, from the preparation and analysis of biological samples to study design and biostatistical analysis, supporting dissemination and publication with capabilities such as multiplexed MALDI imaging.
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Services
Consulting
Custom method development to fit your specific needs
Data interpretation & reporting for meaningful insights
Sample preparation
Standardized protocols for consistency
Advanced extraction methods to preserve key metabolites
Strict quality control to minimize variability
Sample analysis
Comprehensive metabolite profiling with cutting-edge technologies
High-sensitivity detection & structural insights with timsTOF LC-MS & NMR
Spatial metabolomics with high-resolution MALDI imaging
Robust method development to optimize your analysis
Data analysis
Comprehensive & Customizable statistical analysis
State-of-the-art software
Biological pathway analysis
Training and Teaching
Stay tuned for upcoming training programs in metabolomics.
More details coming soon!
Team
Dr. Christoph Trautwein
Head of Core Facility Metabolomics
Dr. Jannik Sprengel
Technical Head
Dr. Mpho Mafata
Data Scientist
Denis Andrei Ispan
Scientist
Sisi Deng
Scientist
Dr. Carolin Schneider
Scientist
Kefan Cheng
Scientist
Paul Klessen
Scientist
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Learn moreEquipment
Sample Preparation
At our Metabolomics Core Facility, we offer specialized sample preparation techniques, including both one-phase and two-phase extractions tailored to different biological matrices and metabolic profiles of interest. To enhance metabolic recovery, Focused Ultrasonication™ is employed, ensuring efficient and reproducible extraction. We process a wide range of sample types, including solid tissues such as stool, brain, and liver, as well as liquid samples like urine, blood serum, cerebrospinal fluid (CSF), and coculture media.
For MALDI imaging, we meticulously prepare cryosectioned samples by applying matrix solutions using a precision sprayer. The choice of matrix depends on the metabolites of interest, optimizing sensitivity and resolution for high-quality spatial metabolomic analysis.
LE220-plus Focused-ultrasonicator
The LE220-plus Focused-ultrasonicator is a scalable, automated and batch-reproducible ultrasound sample preparation instrument. Designed for integration with high-throughput laboratory automation, the LE220-plus includes an integrated AFA ultraCUBE water chiller and a water conditioning module and is used for the extraction of polar metabolites and lipids from solid and semi-solid sample.
HTX TM-Sprayer™
The HTX TM-Sprayer™ is a user-friendly, versatile spraying system that automates sample preparation, ensuring a highly uniform and consistent matrix coating for matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) workflows.
LEICA DM 1000 LED
In the same pipeline we use the LEICA DM 1000 LED to assess spraying performance in the quality control workflow for MALDI imaging. This highly versatile and high-quality microscope is ideal for clinical and laboratory use.
Mass Spectrometer
We employ state-of the-art mass spectrometry to produce high quality data. The principle is based on the formation of charged ions from the analyte molecules.
Compared to classic detection methods such as UV/Vis or RID, mass spectrometry also contains information about the molecular mass (m/z values) and the fragmentation (MS/MS) of the analyzed compounds. In untargeted analyses, this provides useful criteria for identifying compounds, and gain first structural information about unknowns. For targeted approaches, this technique allows for method optimization based on specific structural properties of the analyte, greatly improving specificity and selectivity.
Targeted and Quantitative Analysis
Untargeted and Quantitative Analysis
AB Sciex QTRAP 6500 - Exion UPLC
- Hybrid triple quadrupole-linear ion trap mass spectrometer
- Absolute quantification with very high sensitivity (nM - pM range)
- Suitable for a wide range of sample types
Bruker Impact II qTOF - Elute UPLC and VIP-HESI
- High resolution time-of-flight mass spectrometer (TOF-MS)
- High-throughput studies (24/7 operation) of complex sample matrices (e.g. feces, urine)
- Targeted quantitative panels possible
MALDI Imaging Spatial Metabolomics
4D Lipidomics + 4D Metabolomics
Bruker timsTOF flex MALDI-2 - Elute UPLC
- Combined trapped ion mobility spectrometry (TIMS) and time-of-flight (TOF) technology
- High resolution MALDI imaging of tissues, organoids or cells
- Rapid acquisition rates and high sensitivity
Bruker timsTOF pro 2 - Elute plus UPLC and VIP-HESI
- Combined trapped ion mobility spectrometry (TIMS) and time-of-flight (TOF) technology
- Deep lipid & metabolite profiling of biofluids (e.g. blood serum, urine) and extracts
- Unambiguous separation of isomeric and isobaric metabolites and lipids
FAQ
How do I get started?
If you are interested in our technologies and services, please contact us:
info.cfmet@med.uni-tuebingen.de
Christoph.Trautwein@med.uni-tuebingen.de
