About us

About us

Electron microscopy enables high-resolution analysis of cellular ultrastructure, providing detailed information on cell architecture, content, morphology, and elemental composition that is inaccessible to other imaging techniques. With resolution reaching the sub-nanometer scale, it remains essential for the investigation of subcellular compartments and organelles in basic and translational research. Through analytical electron microscopy, morphological information can be complemented by chemical characterization, including elemental analysis of nanoparticles. Integrated with fluorescence microscopy as a complementary approach, our core facility supports correlative workflows that bridge functional light microscopy and ultrastructural analysis across a wide range of medical and biological research applications.

Services

Consulting


Consultation on the scientific research question


Decision-making on CLSM, EM, or a combined approach


Experimental design, including the selection of antibody panels as well as fixation and sample preparation strategies 

Electron microscopy


Embedding of tissues or cells in Epon or Lowicryl 


Semi-& ultrathin sectioning of embedded samples


Morphological analysis of embedded samples


EFTEM-based elemental analysis


Immunogold labelling of embedded samples


(Immuno-) Negativestaining 

Confocal microscopy


Preparation of Cryo- or Paraffinsections


Immunofluorescence staining 


Confocal Laserscanning Imaging

Post processing


Image and data analysis


Preparation of images to be ready for publication


Illustration of graphs and figures

Team

Dr. Jule Riebelmann

Head of Core Facility Electron Microscopy

Prof. Dr. Martin Schaller

Deputy Head of Core Facility Electron Microscopy

Anneli Yilmaz

Technical Head

Equipment






- High-resolution TEM imaging with a spatial resolution up to 0.34 nm and an information limit of 0.20 nm

- Wide magnification range for both low-magnification overviews and high-resolution structural studies

- Analytical capabilities including electron energy loss spectroscopy (EELS) and electron spectroscopic imaging (ESI)





      - Multi-color laser scanning confocal imaging powered by four diode lasers for flexible and precise fluorescence excitation

      - Airyscan module that maximizes signal collection to enhance resolution, sensitivity, and spatial detail

      - Advanced imaging workflows: high quality 3D Z-stacks, colocalization analysis, large-area tiling, and quantitative image analysis using Zeiss ZEN software

      - Extensive antibody portfolio, with more than 400 antibodies established and validated for confocal imaging

FAQ

    Please contact us via email with a brief description of your project and the required services. We will then arrange a project meeting to discuss the details.
    Following the initial project meeting, we will prepare a cost estimate for the required services. For the full price list please write to
    CoF-EM@med.uni-tuebingen.de.