Our Core Facility Metabolomics operates across three laboratories equipped with state-of-the-art instrumentation for targeted, untargeted, quantitative, as well as spatial metabolomics and lipidomics. We offer comprehensive services, from the preparation and analysis of biological samples to study design and biostatistical analysis, supporting dissemination and publication with capabilities such as multiplexed MALDI imaging.
Custom method development to fit your specific needs
Standardized protocols for consistency
Comprehensive metabolite profiling with cutting-edge technologies
Comprehensive & Customizable statistical analysis
Stay tuned for upcoming training programs in metabolomics.
More details coming soon!
Dr. Christoph Trautwein
Head of Core Facility Metabolomics
Dr. Jannik Sprengel
Technical Head
Dr. Mpho Mafata
Data Scientist
Denis Andrei Ispan
Scientist
Sisi Deng
Scientist
Dr. Carolin Schneider
Scientist
Dr. Gulyaim Sagandykova
Scientist
Kefan Cheng
Scientist
Paul Klessen
Scientist
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Learn moreAt our Metabolomics Core Facility, we offer specialized sample preparation techniques, including both one-phase and two-phase extractions tailored to different biological matrices and metabolic profiles of interest. To enhance metabolic recovery, Focused Ultrasonication™ is employed, ensuring efficient and reproducible extraction. We process a wide range of sample types, including solid tissues such as stool, brain, and liver, as well as liquid samples like urine, blood serum, cerebrospinal fluid (CSF), and coculture media.
For MALDI imaging, we meticulously prepare cryosectioned samples by applying matrix solutions using a precision sprayer. The choice of matrix depends on the metabolites of interest, optimizing sensitivity and resolution for high-quality spatial metabolomic analysis.
The LE220-plus Focused-ultrasonicator is a scalable, automated and batch-reproducible ultrasound sample preparation instrument. Designed for integration with high-throughput laboratory automation, the LE220-plus includes an integrated AFA ultraCUBE water chiller and a water conditioning module and is used for the extraction of polar metabolites and lipids from solid and semi-solid sample.
The HTX TM-Sprayer™ is a user-friendly, versatile spraying system that automates sample preparation, ensuring a highly uniform and consistent matrix coating for matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) workflows.
In the same pipeline we use the LEICA DM 1000 LED to assess spraying performance in the quality control workflow for MALDI imaging. This highly versatile and high-quality microscope is ideal for clinical and laboratory use.
We employ state-of the-art mass spectrometry to produce high quality data. The principle is based on the formation of charged ions from the analyte molecules.
Compared to classic detection methods such as UV/Vis or RID, mass spectrometry also contains information about the molecular mass (m/z values) and the fragmentation (MS/MS) of the analyzed compounds. In untargeted analyses, this provides useful criteria for identifying compounds, and gain first structural information about unknowns. For targeted approaches, this technique allows for method optimization based on specific structural properties of the analyte, greatly improving specificity and selectivity.
- Hybrid triple quadrupole-linear ion trap mass spectrometer
- Absolute quantification with very high sensitivity (nM - pM range)
- Suitable for a wide range of sample types
- High resolution time-of-flight mass spectrometer (TOF-MS)
- High-throughput studies (24/7 operation) of complex sample matrices (e.g. feces, urine)
- Targeted quantitative panels possible
- Combined trapped ion mobility spectrometry (TIMS) and time-of-flight (TOF) technology
- High resolution MALDI imaging of tissues, organoids or cells
- Rapid acquisition rates and high sensitivity
- Combined trapped ion mobility spectrometry (TIMS) and time-of-flight (TOF) technology
- Deep lipid & metabolite profiling of biofluids (e.g. blood serum, urine) and extracts
- Unambiguous separation of isomeric and isobaric metabolites and lipids
If you are interested in our technologies and services, please contact us:
projects.cfmet@med.uni-tuebingen.de
Christoph.Trautwein@med.uni-tuebingen.de
