General Questions

Our service is available to all departments of the UKT as well as to researchers outside the University.

You are welcome to contact us to request information about training, appointments etc.

Fcf-berg@med.uni-tuebingen.de

Phone:      07071 29-82745 (office)

                             29-82835 (Lab)

                             29-68823 (Instrument Lab)

                             29-81979 (Sort Lab)

                  

Register for a user account and add a billing account on the PPMS Management System 

We provide training on an individual basis.  

Training is usually divided into 3 Sessions á 2 hours for Cell analyzers.
Based on that, the usage of the CYTEK™ Aurora requires only one additional “hands-on-training”.
After the training, the participants receive a Certificate of attendance

Instrument training for Cell Analyzers and CYTEK™ Aurora is mandatory for scheduling appointments via PPMS.  

Sorts and Image Stream measurements will be performed by the Facility staff. 

Please contact our Facility staff for a training appointment. 

fcf-berg@med.uni-tuebingen.de

Yes. To maintain the quality of our service you are required to be acquainted with our facility standards as well as our terms and conditions. If the user can provide fundamental flow cytometry knowledge already, the 3 training sessions may be summarized into one session only.

In order to book an appointment you need to have a user account on our online booking system PPMS.

https://ppms.eu/tuebingen

For sort appointments: If you already have an account on PPMS, simply go to the online calendar and book your time window of choice.

A booked session can be cancelled free of charge until 24 hours before the actual starting time. 

Thereafter, the usage fees will apply to our late cancellation policy (see Charges and Fees). If a user does not show up through the entire booked session, usage fees will be calculated according to booked time.

All sorts are performed by our Facility staff.

It’s your data. Please take it with you, we recommend storing your data on multiple places. The Facility keeps only temporary copies of flow cytometry files.

Our Facility labs provide environment for working with genetically modified organisms at biosafety level 2 (S2). The general rules and guidelines apply.

Other Questions

Please bring an unlabeled sample plus single staining controls to properly set up the experiment.

It’s essential to filter the cells through a 40µm cell strainer prior measurement. 

For more detailed information follow our sample guidelines for Cell sorting.

In general, any protocols used for flow cytometry will work with the Image Stream. 

Use 1,5ml polypropylene tubes to process samples.

Ideally, prepare your sample at 20Mio/ml in 50ul.(= ca 1Mio/50µl)

Lower concentration will increase data collection time. 

Please follow our sample Prep Guide

Beside an unlabeled sample and single staining controls we recommend to work with FMOs (Fluorescence minus one) in order to set up the experiment properly.

In principle, you can either use beads or cells. 

Usage of beads is recommended if cell number is limiting, the antigen is dimly expressed or the positive population is rare.

Reference control samples for the CYTEK Aurora need to be treated in the exact same way as your experimental samples in order to get a clean and accurate signature of the fluorophore. 

For Image Stream experiments, cells are recommended for more accurate calculation of the compensation matrix.

It is required to filter your samples to avoid clogging. For sorts it’s mandatory!

See fees for current rates