Institut für Medizinische Virologie und Epidemiologie der Viruskrankheiten

610

Adresse: Elfriede-Aulhorn-Str. 6
72076 Tübingen


Telefonnummer: 07071 29-80247


Faxnummer: 07071 29-5790


E-Mail-Adresse: sekretariat.​iftner@​med.​uni-​tuebingen.​de


Research Stubenrauch Lab

Research Stubenrauch Lab

Clinical relevance of HPV

Persistent infections with high risk HPV from the genus alpha such as HPV16 or HPV31 are causally linked to the development of ano-genital and oro-pharyngeal cancers. In contrast, HPV from the genus beta have been implicated in the development of non-melanoma skin cancer in conjunction with UV exposure.

Contact

Head

frontend.sr-only_#{element.icon}: Prof. Dr. Frank Stubenrauch


frontend.sr-only_#{element.icon}: +49 7071 29-81553


E-Mail-Adresse: frank.stubenrauch@med.uni-tuebingen.de


The HPV replication cycle

HPV infect and complete their replication cycle in keratinocyte cells that are the main constituents of mucosal and cutaneous epithelium. In basal epithelial cells low levels of HPV genome replication and expression of early viral gene products can be observed. Production of infectious HPV occurs only in the upper layers of the infected epithelium. 

  • We aim to understand the regulation of the different phases of the HPV replication cycle in order to identify key regulatory events that can be targeted by antivirals. 
  • We also aim to understand the differences between high risk HPV and beta-HPV

The HPV genome is a double-stranded, covalently closed DNA molecule of 8kbp that is packaged in a chromatinized form. The viral E1 and E2 proteins are the key replication activators and form a complex that recognizes the viral origin. E1 acts as DNA helicase and recruits host cell proteins to initiate the replication of the genome. E2 is not only a loader for the E1 helicase but also modulates viral transcription and acts as segregation factor for the viral genomes upon cell division.

Our studies have uncovered that HPV encode E8^E2, an alternative E2 protein, limits viral replication in undifferentiated cells by repressing viral transcription and E1/E2-dependent DNA replication. Using a panel of different technologies (immunoprecipitation-mass spectrometry, fluorescence microscopy, RNA interference, transcription and replication reporter assays), we have identified the cellular NCoR/SMRT repressor complex as a mediator of E8^E2´s repression activities. Our recent in vivo studies have revealed that E8^E2 prevents the switch to the productive phase in undifferentiated cells which is incompatible with cell division and therefore tumor formation in vivo. This suggests that the E8^E2-NCoR/SMRT complex interaction might be an antiviral target.

  • we investigate the molecular repression mechanism by the E8^E2-NCoR/SMRT complex
  • we investigate the regulation of E2 and E8^E2 proteins by post-transcriptional mechanisms such as post-translational modifications (phosphorylation, acetylation, etc.) in the non-productive and productive phase of the viral replication cycle
  • we investigate the interactome of E2 proteins involved in transcription control, activation of DNA replication, and genome partitioning

Beta-HPV

Beta-HPV have been implicated in the development of non-melanoma skin cancerHowever, in contrast to high risk HPV, the presence of beta-HPV is not required to maintain the cancer phenotype which has led to the model that beta-HPV act by a “hit-and run” mechanism. The beta-HPV E6 and E7 oncoproteins interfere with DNA damage and repair mechanisms which in combination with UV irradiation enhances mutation rates and, thus, ultimately cancer. How beta-HPV E6 and E7 contribute to viral replication has not been explored. We have recently established that normal human keratinocytes support the complete beta-HPV replication cycle.

  • We use this model system to explore the oncogenic activities of E6 and E7 and their contribution to viral replication

Publications

Selected Publications:

    • A splice donor in E6 influences keratinocyte immortalization by beta-HPV49. Rehm TM, Iftner T, Stubenrauch F. J Virol. 2025 Feb 25;99(2):e0164024. doi: 10.1128/jvi.01640-24.
    • Mus musculus papillomavirus 1 E8^E2 represses expression of late protein E4 in basal-like keratinocytes via NCoR/SMRT-HDAC3 co-repressor complexes to enable wart formation in vivo. Kuehner F, Wong M, Straub E, Doorbar J, Iftner T, Roden RBS, Stubenrauch F. mBio. 2023 Aug 31;14(4):e0069623. doi: 10.1128/mbio.00696-23.
    • Transcription Properties of Beta-HPV8 and HPV38 Genomes in Human Keratinocytes. Rehm TM, Straub E, Forchhammer S, Leiter U, Iftner T, Stubenrauch F. J Virol. 2022 Dec 14;96(23):e0149822. doi: 10.1128/jvi.01498-22.
    • Human Papillomavirus 42 Drives Digital Papillary Adenocarcinoma and Elicits a Germ Cell-like Program Conserved in HPV-Positive Cancers. Leiendecker L, Neumann T, Jung PS, Cronin SM, Steinacker TL, Schleiffer A, Schutzbier M, Mechtler K, Kervarrec T, Laurent E, Bachiri K, Coyaud E, Murali R, Busam KJ, Itzinger-Monshi B, Kirnbauer R, Cerroni L, Calonje E, Rütten A, Stubenrauch F, Griewank KG, Wiesner T, Obenauf AC. Cancer Discov. 2023 Jan 9;13(1):70-84. doi: 10.1158/2159-8290.CD-22-0489.
    • Restriction of viral gene expression and replication prevents immortalization of human keratinocytes by a beta-human papillomavirus. Rehm TM, Straub E, Iftner T, Stubenrauch F. Proc Natl Acad Sci U S A. 2022 Mar 15;119(11):e2118930119. doi: 10.1073/pnas.2118930119.
    • Expression of E8^E2 is Required for Wart Formation by Mouse Papillomavirus 1 in vivo. Stubenrauch F, Straub E, Klein K, Kramer D, Iftner T, Wong M, Roden RBS. J Virol. 2021 Mar 25;95(8):e01930-20. doi: 10.1128/JVI.01930-20.
    • Interaction of NCOR/SMRT Repressor Complexes with Papillomavirus E8^E2C Proteins Inhibits Viral Replication. Dreer M, Fertey J, van de Poel S, Straub E, Madlung J, Macek B, Iftner T, Stubenrauch F. PLoS Pathog. 2016 Apr 11;12(4):e1005556.

Zertifikate und Verbände