The M3 Research Center
Malignome, Metabolome and Microbiome

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574

Adresse: Otfried-Müller-Straße 37
72076 Tübingen

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Telefonnummer: +49 7071 29-82518
Michael Lehmann

E-Mail-Adresse: m3-office@med.uni-tuebingen.de

Synthetic biology and antibiotic research

The mission of the Link Lab is to advance knowledge and innovation in synthetic biology and antibiotic research, addressing two global challenges: sustainable production of chemicals and combating antibiotic resistance.

In the realm of antibiotic research, we investigate how bacterial metabolism influences the effectiveness of antibiotics. Through the integration of genomics, proteomics, and metabolomics, we map and explore the mechanisms of antibiotic resistance and antibiotic killing in three of the most critical pathogenic bacteria: E. coli, S. aureus and P. aeruginosa. 

In synthetic biology, our objective is to engineer bacterial metabolism for a sustainable bioeconomy by producing chemicals with non-growing bacteria. Therefore, we employ innovative techniques such as synthetic feedback regulation, thermo-switches and real-time metabolomics to control the growth and overproduction of chemicals in engineered bacteria. Moreover, we aim to utilize synthetic CO2-fixation to access sustainable feedstocks for chemical production.

Portrait

Prof. Dr. Hannes Link

Head of the research group

Hannes Link is a W3 Professor and leads the Bacterial Metabolomics Group at the University of Tübingen. Previously, he served as an Independent Research Group at the Max Planck Institute for Terrestrial Microbiology in Marburg (Germany), and was a Postdoctoral Associate in the Uwe Sauer Lab at the Institute of Molecular Systems Biology, ETH Zurich (Switzerland). Hannes Link holds a Ph.D. in Biochemical Engineering from the Technical University Munich, Germany. His doctoral research focused on "Metabolic control analysis of bioprocesses" under the supervision of Prof. Dirk Weuster-Botz. He also holds a Diploma in Chemical Engineering from the same university. He received an ERC Starting Grant for the MapMe project, which aimed to map metabolic regulators at a genome-scale, as well as a DFG Emmy Noether Fellowship, focusing on bioprocess engineering through dynamic control of metabolic pathways.

Interview
Synthetic biology and antibiotic research – Interview with Prof. Dr. Hannes Link

Join us as we talk to Prof. Dr. Hannes Link, head of the Research Lab. Learn how the team is reprogramming bacteria to combat antibiotic resistance and produce sustainable chemicals – with innovative tools like thermo-switches and synthetic CO₂ fixation.

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Teamfoto
Our research team
  • Board member of the Cluster of Excellence “Controlling microbes to fight infections”, University of Tübingen, Germany
  • Co-director of the Interfaculty Institute for Microbiology and Infection Medicine
  • Steering Committee of the Synthetic Biology Work Group of the DECHEMA
  • Scientific Advisory Board, The German Association for Synthetic Biology (GASB)
map
mechanisms of antibiotic resistance
CO2
-fixation
engineer
bacterial metabolism

Selected publications

  • The SPFH complex HflK-HflC regulates aerobic respiration in bacteria

    Pérez-López MI, Lubrano P, Angelidou G, Hoch S, Glatter T, Paczia N, Link H, Sourjik V. The SPFH complex HflK-HflC regulates aerobic respiration in bacteria. PLoS Biol. 2025 Apr 7;23(4):e3003077. https://doi.org/10.1371/journal.pbio.3003077

  • (p)ppGpp-mediated GTP homeostasis ensures survival and antibiotic tolerance of Staphylococcus aureus

    Salzer A, Ingrassia S, Iyer P, Sauer L, Rapp J, Dobritz R, Müller J, Link H, Wolz C. (p)ppGpp-mediated GTP homeostasis ensures survival and antibiotic tolerance of Staphylococcus aureus. Commun Biol. 2025 Mar 28;8(1):508. https://doi.org/10.1038/s42003-025-07910-6

  • Unveiling the substrate specificity of the ABC transporter Tba and its role in glycopeptide biosynthesis

    Gericke N, Beqaj D, Kronenberger T, Kulik A, Gavriilidou A, Franz-Wachtel M, Schoppmeier U, Harbig T, Rapp J, Grin I, Ziemert N, Link H, Nieselt K, Macek B, Wohlleben W, Stegmann E, Wagner S. Unveiling the substrate specificity of the ABC transporter Tba and its role in glycopeptide biosynthesis. iScience. 2025 Mar 3;28(4):112135. https://doi.org/10.1016/j.isci.2025.112135

  • Metabolic mutations reduce antibiotic susceptibility of E. coli by pathway-specific bottlenecks

    Lubrano P, Smollich F, Schramm T, Lorenz E, Alvarado A, Eigenmann SC, Stadelmann A, Thavapalan S, Waffenschmidt N, Glatter T, Hoffmann N, Müller J, Peter S, Drescher K, Link H. Metabolic mutations reduce antibiotic susceptibility of E. coli by pathway-specific bottlenecks. Mol Syst Biol. 2025 Mar;21(3):274-293. doi: 10.1038/s44320-024-00084-z. Epub 2025 Jan 2. PMID: 39748127; PMCID: PMC11876631. https://doi.org/10.1038/s44320-024-00084-z

  • Metabolite-level regulation of enzymatic activity controls awakening of cyanobacteria from metabolic dormancy

    Doello S, Sauerwein J, von Manteuffel N, Burkhardt M, Neumann N, Appel J, Rapp J, Just P, Link H, Gutekunst K, Forchhammer K. Metabolite-level regulation of enzymatic activity controls awakening of cyanobacteria from metabolic dormancy. Curr Biol. 2025 Jan 6;35(1):77-86.e4. https://doi.org/10.1016/j.cub.2024.11.011

  • Intact protein barcoding enables one-shot identification of CRISPRi strains and their metabolic state

    Pahl V, Lubrano P, Troßmann F, Petras D, Link H. Intact protein barcoding enables one-shot identification of CRISPRi strains and their metabolic state. Cell Rep Methods. 2024 Dec 16;4(12):100908. doi: 10.1016/j.crmeth.2024.100908. Epub 2024 Nov 26. PMID: 39603242; PMCID: PMC11704613. https://doi.org/10.1016/j.crmeth.2024.100908

  • Design of microbial catalysts for two-stage processes

    Shabestary K, Klamt S, Link H, Mahadevan R, Steuer R, Hudson EP. Design of microbial catalysts for two-stage processes.Nat Rev Bioeng 2, 1039–1055 (2024). https://doi.org/10.1038/s44222-024-00225-x

  • Integrating research on bacterial pathogens and commensals to fight infections-an ecological perspective

    Maier L, Stein-Thoeringer C, Ley RE, Brötz-Oesterhelt H, Link H, Ziemert N, Wagner S, Peschel A. Integrating research on bacterial pathogens and commensals to fight infections-an ecological perspective. Lancet Microbe. 2024 Aug;5(8):100843. doi: 10.1016/S2666-5247(24)00049-1. Epub 2024 Apr 9. PMID: 38608681. https://doi.org/10.1016/s2666-5247(24)00049-1

  • DarA-the central processing unit for the integration of osmotic with potassium and amino acid homeostasis in Bacillus subtilis

    Warneke R, Herzberg C, Weiß M, Schramm T, Hertel D, Link H, Stülke J. DarA-the central processing unit for the integration of osmotic with potassium and amino acid homeostasis in Bacillus subtilis. J Bacteriol. 2024 Jul 25;206(7):e0019024. doi: 10.1128/jb.00190-24. Epub 2024 Jun 4. PMID: 38832794; PMCID: PMC11270874. https://doi.org/10.1128/jb.00190-24

  • Metabolic rewiring enables ammonium assimilation via a non-canonical fumarate-based pathway

    Mardoukhi MSY, Rapp J, Irisarri I, Gunka K, Link H, Marienhagen J, de Vries J, Stülke J, Commichau FM. Mardoukhi MSY, Rapp J, Irisarri I, Gunka K, Link H, Marienhagen J, de Vries J, Stülke J, Commichau FM. Metabolic rewiring enables ammonium assimilation via a non-canonical fumarate-based pathway.Microb Biotechnol. 2024 Mar;17(3):e14429. https://doi.org/10.1111/1751-7915.14429

  • Autotrophic growth of Escherichia coli is achieved by a small number of genetic changes

    Ben Nissan R, Milshtein E, Pahl V, de Pins B, Jona G, Levi D, Yung H, Nir N, Ezra D, Gleizer S, Link H, Noor E, Milo R. Autotrophic growth of Escherichia coli is achieved by a small number of genetic changes. Elife. 2024 Feb 21;12:RP88793. https://doi.org/10.7554/elife.88793

  • Mobile CRISPRi moves through the complexity of bacterial genetics

    Lo Presti L, Link H. Mobile CRISPRi moves through the complexity of bacterial genetics. Cell Rep Methods. 2024 Jan 22;4(1):100697. https://doi.org/10.1016/j.crmeth.2024.100697

  • Genomic adaptation of Burkholderia anthina to glyphosate uncovers a novel herbicide resistance mechanism

    Schwedt I, Collignon M, Mittelstädt C, Giudici F, Rapp J, Meißner J, Link H, Hertel R, Commichau FM. Genomic adaptation of Burkholderia anthina to glyphosate uncovers a novel herbicide resistance mechanism. Environ Microbiol Rep. 2023 Dec;15(6):727-739. doi: 10.1111/1758-2229.13184. Epub 2023 Jun 13. PMID: 37311711; PMCID: PMC10667639. https://doi.org/10.1111/1758-2229.13184

  • Mapping temperature-sensitive mutations at a genome scale to engineer growth switches in Escherichia coli

    Schramm T, Lubrano P, Pahl V, Stadelmann A, Verhülsdonk A, Link H. Mapping temperature-sensitive mutations at a genome scale to engineer growth switches in Escherichia coli. Mol Syst Biol. 2023 Oct 12;19(10):e11596. doi: 10.15252/msb.202311596. Epub 2023 Aug 29. PMID: 37642940; PMCID: PMC10568205. https://doi.org/10.15252/msb.202311596

  • Metabolic engineering of the shikimate pathway in Amycolatopsis strains for optimized glycopeptide antibiotic production

    Goldfinger V, Spohn M, Rodler JP, Sigle M, Kulik A, Cryle MJ, Rapp J, Link H, Wohlleben W, Stegmann E. Metabolic engineering of the shikimate pathway in Amycolatopsis strains for optimized glycopeptide antibiotic production. Metab Eng. 2023 Jul;78:84-92. doi: 10.1016/j.ymben.2023.05.005. Epub 2023 May 25. PMID: 37244369. https://doi.org/10.1016/j.ymben.2023.05.005

  • Helicobacter pylori modulates heptose metabolite biosynthesis and heptose-dependent innate immune host cell activation by multiple mechanisms

    Hauke M, Metz F, Rapp J, Faass L, Bats SH, Radziej S, Link H, Eisenreich W, Josenhans C. Helicobacter pylori Modulates Heptose Metabolite Biosynthesis and Heptose-Dependent Innate Immune Host Cell Activation by Multiple Mechanisms. Microbiol Spectr. 2023 Jun 15;11(3):e0313222. doi: 10.1128/spectrum.03132-22. Epub 2023 Apr 27. PMID: 37129481; PMCID: PMC10269868. https://doi.org/10.1128/spectrum.03132-22

  • Dynamic fluctuations in a bacterial metabolic network

    Bi S, Kargeti M, Colin R, Farke N, Link H, Sourjik V. Dynamic fluctuations in a bacterial metabolic network. Nat Commun. 2023 Apr 15;14(1):2173. https://doi.org/10.1038/s41467-023-37957-0

  • Systematic analysis of in-source modifications of primary metabolites during flow-injection time-of-flight mass spectrometry

    Farke N, Schramm T, Verhülsdonk A, Rapp J, Link H. Systematic analysis of in-source modifications of primary metabolites during flow-injection time-of-flight mass spectrometry. Anal Biochem. 2023 Mar 1;664:115036. doi: 10.1016/j.ab.2023.115036. Epub 2023 Jan 7. PMID: 36627043; PMCID: PMC9902335. https://doi.org/10.1016/j.ab.2023.115036

  • Horizontal Transfer of Bacteriocin Biosynthesis Genes Requires Metabolic Adaptation To Improve Compound Production and Cellular Fitness

    Krauss S, Harbig TA, Rapp J, Schaefle T, Franz-Wachtel M, Reetz L, Elsherbini AMA, Macek B, Grond S, Link H, Nieselt K, Krismer B, Peschel A, Heilbronner S. Horizontal Transfer of Bacteriocin Biosynthesis Genes Requires Metabolic Adaptation To Improve Compound Production and Cellular Fitness. Microbiol Spectr. 2023 Feb 14;11(1):e0317622. doi: 10.1128/spectrum.03176-22. Epub 2022 Dec 6. PMID: 36472430; PMCID: PMC9927498. https://doi.org/10.1128/spectrum.03176-22

  • How to deal with toxic amino acids: the bipartite AzlCD complex exports histidine in Bacillus subtilis

    Meißner J, Schramm T, Hoßbach B, Stark K, Link H, Stülke J. How To Deal with Toxic Amino Acids: the Bipartite AzlCD Complex Exports Histidine in Bacillus subtilis. J Bacteriol. 2022 Dec 20;204(12):e0035322. doi: 10.1128/jb.00353-22. Epub 2022 Nov 15. PMID: 36377869; PMCID: PMC9765041. https://doi.org/10.1128/jb.00353-22

  • L-Proline synthesis mutants of Bacillus subtilis overcome osmotic sensitivity by genetically adapting L-arginine metabolism

    Stecker D, Hoffmann T, Link H, Commichau FM, Bremer E. L-Proline Synthesis Mutants of Bacillus subtilis Overcome Osmotic Sensitivity by Genetically Adapting L-Arginine Metabolism. Front Microbiol. 2022 Jun 16;13:908304. https://doi.org/10.3389/fmicb.2022.908304

  • Homeostasis of the biosynthetic E. coli metabolome

    Radoš D, Donati S, Lempp M, Rapp J, Link H. Homeostasis of the biosynthetic E. coli metabolome. iScience. 2022 Jun 2;25(7):104503. https://doi.org/10.1016/j.isci.2022.104503

  • Deciphering the physiological response of Escherichia coli under high ATP demand

    Boecker S, Slaviero G, Schramm T, Szymanski W, Steuer R, Link H, Klamt S. Deciphering the physiological response of Escherichia coli under high ATP demand. Mol Syst Biol. 2021 Dec;17(12):e10504. https://doi.org/10.15252/msb.202110504

  • Spatial alanine metabolism determines local growth dynamics of Escherichia coli colonies

    Díaz-Pascual F, Lempp M, Nosho K, Jeckel H, Jo JK, Neuhaus K, Hartmann R, Jelli E, Hansen MF, Price-Whelan A, Dietrich LE, Link H, Drescher K. Spatial alanine metabolism determines local growth dynamics of Escherichia coli colonies. Elife. 2021 Nov 9;10:e70794. https://doi.org/10.7554/elife.70794

  • Metabolic engineering of Corynebacterium glutamicum for production of UDP-N-acetylglucosamine

    Gauttam R, Desiderato CK, Radoš D, Link H, Seibold GM, Eikmanns BJ. Metabolic Engineering of Corynebacterium glutamicum for Production of UDP-N-Acetylglucosamine. Front Bioeng Biotechnol. 2021 Sep 23;9:748510. https://doi.org/10.3389/fbioe.2021.748510

  • Metabolome and proteome analyses reveal transcriptional misregulation in glycolysis of engineered E. coli

    Wang CY, Lempp M, Farke N, Donati S, Glatter T, Link H. Metabolome and proteome analyses reveal transcriptional misregulation in glycolysis of engineered E. coli. Nat Commun. 2021 Aug 13;12(1):4929. https://doi.org/10.1038/s41467-021-25142-0

  • Mass spectrometry-based metabolomics: a guide for annotation, quantification, and best reporting practices

    Alseekh S, Aharoni A, Brotman Y, Contrepois K, D'Auria J, Ewald J, C Ewald J, Fraser PD, Giavalisco P, Hall RD, Heinemann M, Link H, Luo J, Neumann S, Nielsen J, Perez de Souza L, Saito K, Sauer U, Schroeder FC, Schuster S, Siuzdak G, Skirycz A, Sumner LW, Snyder MP, Tang H, Tohge T, Wang Y, Wen W, Wu S, Xu G, Zamboni N, Fernie AR. Mass spectrometry-based metabolomics: a guide for annotation, quantification and best reporting practices. Nat Methods. 2021 Jul;18(7):747-756. doi: 10.1038/s41592-021-01197-1. Epub 2021 Jul 8. PMID: 34239102; PMCID: PMC8592384. https://doi.org/10.1038/s41592-021-01197-1

  • C4-dicarboxylates and L-aspartate utilization by Escherichia coli K-12 in the mouse intestine

    Schubert C, Winter M, Ebert-Jung A, Kierszniowska S, Nagel-Wolfrum K, Schramm T, Link H, Winter S, Unden G. C4-dicarboxylates and l-aspartate utilization by Escherichia coli K-12 in the mouse intestine: l-aspartate as a major substrate for fumarate respiration and as a nitrogen source. Environ Microbiol. 2021 May;23(5):2564-2577. doi: 10.1111/1462-2920.15478. Epub 2021 May 4. PMID: 33754467. https://doi.org/10.1111/1462-2920.15478

  • Multi-omics analysis of CRISPRi-knockdowns identifies mechanisms that buffer decreases of enzymes in Escherichia coli metabolism

    Donati S, Kuntz M, Pahl V, Farke N, Beuter D, Glatter T, Gomes-Filho JV, Randau L, Wang CY, Link H. Multi-omics Analysis of CRISPRi-Knockdowns Identifies Mechanisms that Buffer Decreases of Enzymes in E. coli Metabolism. Cell Syst. 2021 Jan 20;12(1):56-67.e6. doi: 10.1016/j.cels.2020.10.011. Epub 2020 Nov 24. PMID: 33238135. https://doi.org/10.1016/j.cels.2020.10.011

  • Systematic alteration of in vitro metabolic environments reveals empirical growth relationships in cancer cell phenotypes,

    Kochanowski K, Sander T, Link H, Chang J, Altschuler SJ, Wu LF. Systematic alteration of in vitro metabolic environments reveals empirical growth relationships in cancer cell phenotypes. Cell Rep. 2021 Jan 19;34(3):108647. https://doi.org/10.1016/j.celrep.2020.108647

  • Metabolism of non-growing bacteria

    Lempp M, Lubrano P, Bange G, Link H. Metabolism of non-growing bacteria. Biol Chem. 2020 Nov 26;401(12):1479-1485. https://doi.org/10.1515/hsz-2020-0201

  • High-throughput enrichment of temperature-sensitive argininosuccinate synthetase for two-stage citrulline production in Escherichia coli

    Schramm T, Lempp M, Beuter D, Sierra SG, Glatter T, Link H. High-throughput enrichment of temperature-sensitive argininosuccinate synthetase for two-stage citrulline production in E. coli. Metab Eng. 2020 Jul;60:14-24. doi: 10.1016/j.ymben.2020.03.004. Epub 2020 Mar 13. PMID: 32179161; PMCID: PMC7225747. https://doi.org/10.1016/j.ymben.2020.03.004

  • Growth-rate dependent resource investment in bacterial motile behavior quantitatively follows the potential benefit of chemotaxis

    Ni B, Colin R, Link H, Endres RG, Sourjik V. Growth-rate dependent resource investment in bacterial motile behavior quantitatively follows potential benefit of chemotaxis. Proc Natl Acad Sci U S A. 2020 Jan 7;117(1):595-601. doi: 10.1073/pnas.1910849117. Epub 2019 Dec 23. PMID: 31871173; PMCID: PMC6955288. https://doi.org/10.1073/pnas.1910849117

  • Breakdown of Vibrio cholerae biofilm architecture induced by antibiotics disrupts community barrier function

    Díaz-Pascual F, Hartmann R, Lempp M, Vidakovic L, Song B, Jeckel H, Thormann KM, Yildiz FH, Dunkel J, Link H, Nadell CD, Drescher K. Breakdown of Vibrio cholerae biofilm architecture induced by antibiotics disrupts community barrier function. Nat Microbiol. 2019 Dec;4(12):2136-2145. doi: 10.1038/s41564-019-0579-2. Epub 2019 Oct 28. PMID: 31659297; PMCID: PMC6881181. https://doi.org/10.1038/s41564-019-0579-2

  • Systematic identification of metabolites controlling gene expression in E. coli

    Lempp M, Farke N, Kuntz M, Freibert SA, Lill R, Link H. Systematic identification of metabolites controlling gene expression in E. coli. Nat Commun. 2019 Oct 2;10(1):4463. https://doi.org/10.1038/s41467-019-12474-1

  • CRISPRi-based downregulation of transcriptional feedback improves growth and metabolism of arginine overproducing Escherichia coli

    Sander T, Wang CY, Glatter T, Link H. CRISPRi-Based Downregulation of Transcriptional Feedback Improves Growth and Metabolism of Arginine Overproducing Escherichia coli. ACS Synth Biol. 2019 Sep 20;8(9):1983-1990. doi: 10.1021/acssynbio.9b00183. Epub 2019 Aug 27. PMID: 31429546. https://doi.org/10.1021/acssynbio.9b00183

  • Broadening the scope of enforced ATP wasting as a tool for metabolic engineering in Escherichia coli

    Boecker S, Zahoor A, Schramm T, Link H, Klamt S. Broadening the Scope of Enforced ATP Wasting as a Tool for Metabolic Engineering in Escherichia coli. Biotechnol J. 2019 Sep;14(9):e1800438. doi: 10.1002/biot.201800438. Epub 2019 May 17. PMID: 30927494. https://doi.org/10.1002/biot.201800438

  • Quorum sensing and metabolic state of the host control lysogeny-lysis switch of bacteriophage T1

    Laganenka L, Sander T, Lagonenko A, Chen Y, Link H, Sourjik V. Quorum Sensing and Metabolic State of the Host Control Lysogeny-Lysis Switch of Bacteriophage T1. mBio. 2019 Sep 10;10(5):e01884-19. https://doi.org/10.1128/mbio.01884-19

  • Allosteric Feedback Inhibition Enables Robust Amino Acid Biosynthesis in E. coli by Enforcing Enzyme Overabundance

    Sander T, Farke N, Diehl C, Kuntz M, Glatter T, Link H. Allosteric Feedback Inhibition Enables Robust Amino Acid Biosynthesis in E. coli by Enforcing Enzyme Overabundance. Cell Syst. 2019 Jan 23;8(1):66-75.e8. doi: 10.1016/j.cels.2018.12.005. Epub 2019 Jan 9. PMID: 30638812; PMCID: PMC6345581. https://doi.org/10.1016/j.cels.2018.12.005

  • Selective enrichment of slow-growing bacteria in a metabolism-wide CRISPRi library with a TIMER protein

    Beuter D, Gomes-Filho JV, Randau L, Díaz-Pascual F, Drescher K, Link H. Selective Enrichment of Slow-Growing Bacteria in a Metabolism-Wide CRISPRi Library with a TIMER Protein. ACS Synth Biol. 2018 Dec 21;7(12):2775-2782. doi: 10.1021/acssynbio.8b00379. Epub 2018 Nov 16. PMID: 30424596. https://doi.org/10.1021/acssynbio.8b00379

  • Capacity for instantaneous catabolism of preferred and non-preferred carbon sources in Escherichia coli and Bacillus subtilis

    Buffing MF, Link H, Christodoulou D, Sauer U. Capacity for instantaneous catabolism of preferred and non-preferred carbon sources in Escherichia coli and Bacillus subtilis. Sci Rep. 2018 Aug 6;8(1):11760. https://doi.org/10.1038/s41598-018-30266-3

  • Reserve flux capacity in the pentose phosphate pathway enables Escherichia coli’s rapid response to oxidative stress

    Christodoulou D, Link H, Fuhrer T, Kochanowski K, Gerosa L, Sauer U. Reserve Flux Capacity in the Pentose Phosphate Pathway Enables Escherichia coli's Rapid Response to Oxidative Stress. Cell Syst. 2018 May 23;6(5):569-578.e7. doi: 10.1016/j.cels.2018.04.009. Epub 2018 May 9. PMID: 29753645. https://doi.org/10.1016/j.cels.2018.04.009

  • Engineered Production of Short-Chain Acyl-Coenzyme A Esters in Saccharomyces cerevisiae

    Krink-Koutsoubelis N, Loechner AC, Lechner A, Link H, Denby CM, Vögeli B, Erb TJ, Yuzawa S, Jakociunas T, Katz L, Jensen MK, Sourjik V, Keasling JD. Engineered Production of Short-Chain Acyl-Coenzyme A Esters in Saccharomyces cerevisiae.ACS Synth Biol. 2018 Apr 20;7(4):1105-1115. doi: 10.1021/acssynbio.7b00466. Epub 2018 Mar 12. PMID: 29498824. https://doi.org/10.1021/acssynbio.7b00466

  • Crosstalk between transcription and metabolism: how much enzyme is enough for a cell?,

    Donati S, Sander T, Link H. Crosstalk between transcription and metabolism: how much enzyme is enough for a cell? Wiley Interdiscip Rev Syst Biol Med. 2018 Jan;10(1). doi: 10.1002/wsbm.1396. Epub 2017 Aug 15. PMID: 28810056. https://doi.org/10.1002/wsbm.1396

  • Time-optimized isotope ratio LC-MS/MS for high-throughput quantification of primary metabolites

    Guder JC, Schramm T, Sander T, Link H. Time-Optimized Isotope Ratio LC-MS/MS for High-Throughput Quantification of Primary Metabolites.Anal Chem. 2017 Feb 7;89(3):1624-1631. doi: 10.1021/acs.analchem.6b03731. Epub 2017 Jan 13. PMID: 28050903. https://doi.org/10.1021/acs.analchem.6b03731