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Registration

In order to use the FCF services, all users have to create an account via PPMS  to be registered at FCF-Berg and send  the filled User Sheet for Sorts or the User Sheet for Analyzers via email to

fcf-berg@med.uni-tuebingen.de

Link zu PPMS

Cell Sorter

Cell sorting is performed by FCF staff on a BD FACS Aria cell sorter equipped with four lasers (355nm or 402nm, 488nm, 561nm, 640nm excitation wavelengths) and 14 fluorescence channel detection. The cell sorter is able to perform high-speed (up to 20,000 events/sec), high-purity (up to 99.9%) sterile sorts (biosafety level 2) into different types of devices (tubes, plates or slides). The cell sorter is operated by FCF staff during the extended business hours.

SONY MA900 Multi-Application

- 4 laser (405nm, 488nm, 561nm, 640nm)
- 12 fluorescence channel detection
- 4 way sorting
- bulk and single cell sorting
- index sort
- micro fluidic chip-based design: 70µm, 100µm, 130µm
   disposable sorting chips

configuration

operators guide

Analyzer

The FCF is equipped with four advanced benchtop Analyzers , which have up to four lasers (488, 561, 640 and 405 nm excitation wavelengths) and 16 fluorescence channels. Researchers can be trained by the FCF staff in order to use the machines independently without an operator. We also provide detailed advice and support on the choice of fluorophores , equipment and protocols.

Spectral Analyzer

The CYTEK™ Aurora allows separation of fluorochromes with very close emission profiles. This enables the use of a wide range of new fluorochrome combinations. The machine is equipped with  UV, violet, blue, yellow/green and red lasers and provides up to 64 fluorescence channels. A  plate sample loader is part of the assembly. The AURORA is available for  independant use following a training by the FCF staff.

Imaging Cytometrie

Imaging Flow cytometry combines the features of Flow cytometry and Fluorescence microscopy and overcomes the limitations of both techniques. The Image Stream mkII is equipped with violet, blue, yellow/green and red lasers, 12 Image Channels , 2 high sensitive CCD cameras, 3 objective lenses and an EDF tool for extended depths of field. The ISxmkII is operated by the FCF staff. A preliminary consulting is mandatory  to clarify the overall  goal of the experiment or project. We offer advice and support for sample preparation and are happy to perform the data analysis together with the researchers.

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